Life Science Research Professional 2 - 71906
Stanford University is seeking a Life Science Research Professional 2 to perform more complex functions and activities involved in defined research projects, and independently conduct a portion of the research project(s).
About the Department of Pathology
Comprised of extraordinary faculty and staff, our mission is to improve the diagnosis, treatment and basic understanding of human disease. We accomplish this through our clinical services, research, and training the future leaders in pathology and related fields. A major focus of clinical research in the Department continues to be the correlation of patient outcome and treatment response with the surgical pathologic diagnosis of human cancers. Everything we do is to provide the highest quality of pathology diagnostic services to the patients for whom we passionately care.
For more information about the department visit http://pathology.stanford.edu/
About the Position:
We are seeking a Life Science Research Assistant (LRSA) to join the group of Dr. Mike Angelo at the Stanford Blood Center in the Department of Pathology. This LRSA will aid in the development of reagents and methods for a next-generation IHC platform (Multiplexed ion beam imaging) housed in the Angelo lab. A strong background in tissue histology and immunohistochemistry is highly desired. The LSRA will play a key role in the operation and maintenance of the custom instrumentation built for this application. Additionally the LRSA will have skills in fluorescence based flow cytometry as well in basic cell biology and molecular biology methodologies such a PCR, cloning, western blotting, protein conjugation and purification. The LSRA must have basic computer skills and be a good team player. The LRSA will be involved with general lab organization as well as working to support the projects of graduate students and postdoctoral fellows in the lab.
- Perform complex (lab and/or field) experiments for a major portion of the research project. Maintain detailed records of experiments and outcomes.
- Interpret and analyze results, often where few guidelines exist. Based on prior results, contribute to development of new research protocols and procedures or adaptation of existing protocols.
- Make recommendations on plan design and/or research direction to principal investigator.
- Work with health and safety department to ensure lab complies with regulations and all required trainings.
- Participate in multidisciplinary teams across different faculties or schools.
- Oversee supply and equipment budget under supervision of principal investigator or business manager.
- Perform ongoing literature review to remain current with new procedures and related research; present findings for lab work group.
- Contribute substantively to the preparation of papers for publication, especially in the results section. May contribute to publication of findings and present ongoing work and findings to colleagues at academic conferences.
- Supervise (either formally or informally) staff or students as needed, including oversight and instruction on techniques, as well as consultation on project work. Serve as technical resource for other research staff.
- Perform general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. May assist with purchasing laboratory equipment.
* - Other duties may also be assigned
- Bachelors degree in Biochemistry, Analytical Chemistry, Cell Biology, Biological Sciences or related field.
- A minimum of three years of laboratory research experience desired.
- Experience planning and performing immunochemistry and bio-conjugation procedures is highly desired; specifically amine and sulfhydryl specific chemistry for covalently linking ligands to proteins (specifically immunoglobulins).
- Experience and knowledge of planning and performing the following items is highly desired:
- The procedure for purification of the resulting conjugates (size exclusion/ gel filtration/ affinity purification/ other chromatographic approaches).
- Immunofluorescence staining procedures.
- Running a flow cytometer and analyzing flow cytometry data (FlowJo and cytobank.org) as well as other forms of (antibody-based) immuno-assays (ELISA, IP, bead-based assays).
- The principles and operation of mass spectrometry instrumentation particularly time of flight (TOF)-based detection, is highly desired. Hands-on experience operating such equipment would also be an asset.
- Experience and knowledge of planning and performing the following items is desired, but not required:
- Basic aseptic technique with experience performing mammalian cell culture in a BS2 class hood; Experience working with blood and/or bone marrow from mammalian sources; Ability to write scripts (visual basic, Perl, etc.) to mine text data would also be an asset.
- The candidate should be able to work independently, be highly motivated, well organized, adaptable and work well within a highly interdisciplinary and international team. Good communication and interpersonal skills as well as fluent English are essential, as well as proficient computer skills (Mac and PC). Additional desired, but not required, skills:
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field and three years of related experience, or Master's degree.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- Comprehensive understanding of scientific principles.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Strong analytical skills and excellent judgment.
- Ability to work under deadlines with general guidance is essential.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
* - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
Location: School of Medicine